백서 두개골결손모델에서 실크단백과 골형성단백 이식체가 골재생에 미치는 영향

남정훈; 노경록; 방은오; 유우근; 강응선; 권해용; 김성곤; 박영주

SITE LINK

백서 두개골결손모델에서 실크단백과 골형성단백 이식체가 골재생에 미치는 영향
The effect of silk fibroin and rhBMP-2 on bone regeneration in rat calvarial defect model

대한구강악안면외과학회지 2010년 36권 5호 p.366 ~ 374

남정훈, 노경록, 방은오, 유우근, 강응선, 권해용, 김성곤, 박영주,

소속 상세정보

남정훈 ( Nam Jeong-Hun ) - 한림대학교 의과대학 강남성심병원 구강악안면외과학교실
노경록 ( Noh Kyung-Lok ) - 한림대학교 의과대학 강남성심병원 구강악안면외과학교실
방은오 ( Pang Eun-O ) - 한림대학교 의과대학 강남성심병원 구강악안면외과학교실
유우근 ( Yu Woo-Geun ) - 한림대학교 의과대학 강남성심병원 구강악안면외과학교실
강응선 ( Kang Eung-Seon ) - 한림대학교 의과대학 강남성심병원 구강악안면외과학교실
권해용 ( Kweon Hae-Yong ) - 농촌진흥청
김성곤 ( Kim Seong-Gon ) - 강릉원주대학교 치과대학 구강악안면외과학교실
박영주 ( Park Young-Ju ) - 한림대학교 의과대학 강남성심병원 구강악안면외과학교실

KMID : 0355620100360050366

Abstract

Introduction:This study evaluated the capability of silk fibroin (SF) and recombinant human bone morphogenetic protein-2 loaded SF (SF-BMP) as a bone defect replacement matrix when grafted in a calvarial bone defect of rats in vivo.

Materials and Methods: A total 70 calvarial critical size defects (5.0 ㎜ in diameter) made on 35 adult female Sprague-Dawley rats were used in this study. The defects were transplanted with (1) rhBMP-2 loaded silk fibroin graft (SF-BMP: 0.8+10 ㎍), (2) Silk fibroin (SF: 10 ㎍), and (3) no graft material (Raw). The samples were evaluated with soft x-rays, alkaline phosphatase activity, calcium/phosphate quantification, histological and histomorphometric analysis at postoperative 4 and 8 weeks.

Results: The SF-BMP group (48.86±14.92%) had a significantly higher mean percentage bone area than the SF group (24.96±11.01%) at postoperative 4 weeks.(P＜0.05) In addition, the SF-BMP group (40.01±12.43%) had a higher % bone area at postoperative 8 weeks than the SF group (33.26±5.15%). The mean ratio of gray scale levels to the host bone showed that the SF-BMP group (0.67±0.08) had a higher mean ratio level than the SF group (0.61±0.09) at postoperative 8 weeks. These differences were not statistically significant.(P=0.168 and P=0.243, respectively) The ratio of the calcium and phosphate contents of the SF-BMP (0.93±0.22) group was lower than that of the SF (1.90±1.42) group at postoperative 4 weeks. However, the SF-BMP group (0.75±0.31) had a higher Ca/PO₄ ratio than the SF (0.68±0.04) at postoperative 8 weeks. These differences were not statistically significant.(P=0.126 and P=0.627, respectively) For the bone-specific alkaline phosphatase (ALP) activity, which is recognized as a reliable indicator of the osteoblast function, the SF-BMP (23.71±8.60 U/L) groups had a significantly higher value than the SF group (12.65±6.47 U/L) at postoperative 4 weeks.(P＜0.05) At postoperative 8 weeks, the SF-BMP (21.65±10.02 U/L) group had a lower bone-specific ALP activity than the SF group (16.72±7.35 U/L). This difference was not statistically significant.(P=0.263) For the histological evaluation, the SF-BMP group revealed less infla㎜ation, lower foreign body reactions and higher bone healing than the SF group at postoperative 4 and 8 weeks. The SF group revealed more foreign body reactions at postoperative 4 weeks. However, this i㎜unogenic reaction decreased and the remnant of grafted material was observed at postoperative 8 weeks. For histomorphometric analysis, the SF-BMP group had a significantly longer bone length to total length ratio than those of the SF group at postoperative 4 and 8 weeks.(P＜0.05)

Conclusion: The rhBMP-2 loaded silk fibroin graft revealed fewer i㎜unoreactions and infla㎜ation as well as more new bone formation than the pure silk fibroin graft. Therefore, silk fibroin may be a candidate scaffold for tissue engineered bone regeneration.